Emma White

For her PIPS, Jess chose to do something slightly different. She volunteered for Roundabout: South Yorkshire’s biggest youth homelessness charity which supports over 300 16-25 year olds every day. Roundabout provide a range of support from emergency accommodation, to bespoke career advice and tenancy support.

The featured image is of the roundabout logo

What did you do?

I worked for a charity called Roundabout, which is South Yorkshire’s biggest youth homelessness charity, supporting over 300 16-25 year olds, every day. They provide a range of support from emergency accommodation, to bespoke career advice and tenancy support. Because Roundabout is a relatively small, local, charity I got the opportunity to work across multiple different departments, and therefore got a great feeling of the charity overall, and how charities are managed and operate.

Two days a week I worked in ‘Central services’. This is the administrative and financial side of the charity. Here I helped with housing benefit applications, balancing books and working out why some client accounts were in arrears. I also helped in the maintenance of the client database, and learnt about the legislation surrounding trustees.  

Once a week I worked at ‘Homeless prevention services’. This is a drop in service in Sheffield city centre, where young people who are homeless, or need housing advice can come, and get free, unbiased support. The best part about this service is that no young person is ever turned away, the Roundabout staff always find a safe place for them to stay. Here, I shadowed and learnt how to interview young people who presented at the service and learnt about the different streams of support available to homeless young people, either from the council or local charities. I also helped in writing their 2021 National Lottery Annual Report, and the final Children in Need report for Roundabout’s five-year funding cycle. I interpreted data collected by the drop in service and made graphs to best display the charities output and contribution towards preventing youth homelessness in South Yorkshire.  

Twice a week I worked at the Roundabout hostel. Here I improved some of their documentation for recording client information, making it more stream lined and user friendly. I also spent a lot of time getting to know the young people, and supported them in day-to-day tasks. I also independently ran ‘life skill’ sessions where I baked and cooked with the residents, and organised their Christmas party.

In addition to this, I also did some work with the fundraising team. I assisted in the organisation and running of multiple events including the ‘South Yorkshire chocolate festival’, which was attended by 2000 people, a ‘Sleep out’ event, and I also ran a stall at the Sheffield Christmas market educating the public about youth homelessness and the work of Roundabout. Finally, I also got the opportunity to work with the Grants team. I successfully wrote an application to a community grant sponsored by Vodaphone, which was awarded. I also researched and wrote a letter to City Fibre enquiring about obtaining free Wi-Fi for all residents, and applied to a community grant with the South Yorkshire Community Foundation (SYCF), both of which we are yet to hear the outcome of. 

 What made you want to do that particular placement?

I wanted to take this PIPS opportunity to experience something outside of science, and to hopefully make a positive impact on my local community. I also wanted to gain experience and skills that are more easily recognised by companies outside of science and academia, for example in finance, management and teamwork.  

How did you go about finding and planning your PIPS?  

I had already heard of the amazing work being done by Roundabout in South Yorkshire, so I sent them a message on the ‘Contact us’ portion of their website, describing who I am, the skills I have, and explaining what a PIPS placement entails.

I wasn’t expecting to hear back from them, but they emailed me back almost straight away! I then arranged to meet with them in person and discuss what I could potentially do during my internship. Before this meeting I had made a fresh CV and made a list of things I would like to do during the placement, which really helped steer the meeting and plan what I could be involved in. I then started 2 months later and had the best time!

What have you gained from doing your PIPS?

The biggest thing that I gained from my PIPS was confidence in my abilities. Working in an environment where there were no expectations put on me meant that I really thrived. I have learnt that it’s always best to just give something a go even if you’re not 100 % sure on what you’re doing, it’s better to just try than to not do anything at all!

I gained more confidence in my ability to back myself and confidently put forward ideas. Working with a wide range of people at Roundabout has made me realise that as PhD students we have strong problem-solving skills and often come up with good ideas. It was really nice to be surrounded by other positive, enthusiastic members of staff. I also learnt so much about business and people management. Being an intern and working in so many different teams meant that I saw a lot of different working and management styles. I learnt so much about the general organisation of the charity, the hiring process, and how everyone’s roles fit into the overall running of Roundabout. Very interesting! 

How would you sum up your PIPS experience?

My PIPS has been an amazing time and has made me realise that as PhD students we have a lot more skills than we think we do! I think I have grown a lot as a person. I am so grateful to my PIPS supervisor Geoff for taking me on, and to everyone at Roundabout for making it such a lovely time. Every day was so varied and I genuinely looked forward to going in every day!

What advice would you give to other PGRs about PIPS?

PIPS seem like a really daunting and stressful thing to organise, but in truth they are not, I promise! If you are interested in working in the charity sector I would definitely recommend reaching out to a smaller, local charity rather than a ‘big name’ charity (i.e. CRUK, the NSPCC, Age UK etc). Smaller charities have less infrastructure and are therefore more flexible and more likely to be able to take on a PIPS. Additionally, because they generally don’t take on PhD interns you can really shape what you spend your time doing and you are special because you are the first/only one!

Robert is a 3rd-year PhD student at the University of Sheffield who completed his PIPS placement at ADAS, the UK’s largest independent provider of agricultural and environmental consultancy, policy advice and research and development. He was able to connect to ADAS with the help of LinkedIn, which has proven to be a vital networking tool. The WRDTP has its own LinkedIn group and page for our students to join and follow which we hope will enable more connections such as Robert’s in the future.

The featured image is of the logo for ADAS.

What did you do?

I worked with crop physiology and agronomy consultants at ADAS.

My first task was to review existing methods of analysing data gathered on farms and similar data sets and examine different methods to analyse the data collected over the last 7 years. I delivered the results of this as a presentation to the crop physiology group within ADAS.

The next part of my placement involved working with various consultants with trial carried out with various industrial partners using new products and techniques. This largely involved data preparation and analysis.

What made you want to do that particular placement?

I have long had an interest in the agricultural sector understanding how it works, the improvements that can be made and the changes necessary to move towards a more sustainable future. As a group who work closely with UK growers and the larger companies that support these growers, ADAS was centrally placed to provide insight into these interests.

How did you go about finding and planning your PIPS?

I reached out to a previous researcher at the university of Sheffield via LinkedIn and they helped me to arrange a placement.

What have you gained from doing your PIPS?

I have gained a larger appreciation of agricultural consulting. The skills that are required and the expertise and knowledge necessary. I have a greater appreciation for a range of statistical tools required in this field, some of which are transferable to my PhD work.

Secondly, the requirement to work to strict deadlines with multiple projects running in tandem has allowed the development of my organisation and time keeping skills. As well as a greater appreciation of my own abilities keeping to deadlines and the importance of reasonable expectations, particularly workload.

Finally, I have developed a network of contacts within an industry I am interested in working in after my PhD.

How would you sum up your PIPS experience?

Overall, my PIPS placement was a very positive experience, allowing me to work in an engaging environment and providing me with first-hand experience in an industry that greatly interests me. Not only will the skills I have been able to develop, but the contacts I have made, will undoubtedly provide new potential opportunities after finishing my PhD.

What advice would you give to other PGRs about PIPS?

I would strongly suggest investing time in researching potential roles and industries that might interest you. Linked in is a very powerful tool for reaching out to companies, with will mostly be more than happy to receive enthusiastic and skilled individuals such as those completing a PhD. Even if a role is not advertised, just contacting potential companies is a great way to potentially craft a more bespoke placement that suits what you are trying to get out of the experience.

–

Jake is a PhD student at the University of Leeds who completed his PIPS placement at at Oxitec’s UK facility in Milton Park, Oxfordshire. Oxitec are a company that have been leading the way in the use of genetically modified insects as a tool for targeted pest control in areas of global health and agriculture. The most advanced of their projects is with the mosquito Aedes aegypti that transmits several viral diseases including Dengue, Zika and yellow fever. Oxitec’s Friendly^TM Aedes aegypti have been through field trials in Brazil, Panama, Cayman Islands and Florida, and are even now commercially available direct to consumers in Brazil.

The featured image is of the Molecular Building at Oxitec.

What did you do?

My placement was split into three rotations that gave me a broad overview of the work that is required to develop and maintain Oxitec’s transgenic insects. The first of these rotations was within the molecular lab, where transgene constructs are designed and transgenic individuals are genotyped to assess the viability and safety of candidate strains. The molecular team also support field trials by genotyping trap samples to monitor the spread of the transgene in wild populations. My work primarily involved DNA extractions and a variety of PCR reactions to support these tasks across several projects.

My second month was spent with the mass rearing team whose job it is to scale up insect production for future field release. I was brought into the team during a proof of concept experiment to produce 20,000 soy bean looper adults a week. This is a species of moth whose larval stage represents a serious agricultural pest in the Americas. Much of the day to day work revolved around preparing the diet and plastic housing for the larvae, and harvesting the pupae for adult cages before their eclosion. We also had to contend with consistent drawbacks from mould and viral outbreaks. We ran experiments alongside to try and establish the most efficient conditions for producing the best insect yield.

My final month was with the mosquito strain development team. Here I was performing micro-injections on Anopholes mosquito eggs, attempting to generate transgenic lines. I supported the team in maintaining and penetrance testing new lines, as well as screening the offspring larvae of injected mosquitos for first generation transgenics. The transgene includes a DsRed fluorescent marker so that the transgenic insects can easily be distinguished from their wild type counterparts.

What made you want to do that particular placement?

I wanted to get a taste for R&D work within an industry setting and Oxitec is one of the few companies I came across that could offer this whilst combining two areas of biology that I am particularly interested in, insect biology and genetics.

How did you go about finding and planning your PIPS?

For a while I was scanning through the directory of biotech companies listed in biopharmguy.com. I had reached out to several companies with no luck and then I happened upon a BBC article about Oxitec’s Aedes aegypti field trial in Florida and was immediately drawn to the company’s science and objectives.

After I saw this, I found out that an old post-doc from a partner lab at Leeds was now working there. I reached out to her and she helped bring it all together and became my key host contact throughout. This was very fortunate and reinforced the importance of having good connections.

What have you gained from doing your PIPS?

My time at Oxitec gave me a great insight into an industry R&D setting. Having worked in three different areas of the company and across several different projects at differing stages of development, I gained a clear overview of how the company operates. I was also lucky enough to attend two presentations by the CEO of the company whilst I was there which gave me a great perspective of how the technology was being field tested and rolled out abroad.

As well as an appreciation for the inner workings of the company, I developed a wide range of new research skills. My experience moving between labs also gave me a deeper appreciation for the subtle differences that team dynamics and communication can make in research.

How would you sum up your PIPS experience?

My time working at Oxitec showed me first-hand how my skills can be applied directly to tackling real world problems. The rotations gave me a good variety of experience across several different scientific disciplines and meant I was consistently being challenged over the course of the internship. The PIPS has provided me with a refreshing break from the PhD and I’m now going back to my own project with a new perspective and enthusiasm.

What advice would you give other PGRs about PIPS?

When you are looking, think about where you may have contacts already as this will help you get your foot in the door. That being said, there’s no harm in contacting places regardless of whether you know anyone there. Perhaps most importantly, don’t be afraid to keep asking questions throughout your internship… The better understanding you have of what you are doing, the more you will get out of the experience.

Egle Beigaite is a 4th year PhD student at the University of York who completed her PIPS placement at CasZyme, a biotech company based in Vilnius, Lithuania. CasZyme are an organisation whose research focuses on revolutionising the field of CRISPR-based Molecular Tools.

The featured image is of Egle with her PIPS colleagues.

What did you do?

My project focused on the characterisation of Cas proteins which could be used in genome editing. The techniques that I was exposed to included: colony PCR, protein purification using AKTA system (Ni and Heparin columns for affinity purification), bacterial cell transformation, cloning, RNA, DNA and plasmid purification using minipreps and midi preps. One of the main aims of my project was to determine the optimal growth and expression conditions for bacterial cells, which were then used to purify Cas proteins. I was also introduced to RNA synthesis (in vitro transcription), Cas protein and gRNA complex assembly as well as Cas activity assays.

What made you want to do that particular placement?

While relatively recent, CRISPR genome editing technology is becoming the main workhorse for genome editing in both academia and industry. Dr Gasiunas, who established the company, has previously worked in Prof. Siksnys’s lab and published several key papers in CRISPR research (Gasiunas et al 2012; Karvelis et al 2015; Gasiunas et al 2020). Prof. Siksnys, together with Prof Charpentier and Prof Doudna have received a prestigious Kavli prize for his independent development of CRISPR. I believed that it was the best accessible location for me to deepen my understanding in CRISPR technology and learn new protein characterisation techniques. In addition, this is a start-up company with only 10 employees. I thought that, by starting in such a small company, it would be easier for me to adapt and would also allow me to learn more things.

How did you go about finding and planning your PIPS?

Initially, I picked two countries I believed I could easily find an internship: the Netherlands and Lithuania (I am originally Lithuanian and started search way before COVID began), and researched biotech companies in both countries. After the interviews, I received two offers from Centre for Human Drug Research (CHDR, Netherlands) and CasZyme (Lithuania). Eventually, I chose to go to work for CasZyme as I really wanted to broaden my understanding of CRISPR technology.

Regarding CHDR, my project there would have focused on neurological conditions, which is what my PhD project is about, so I found it less interesting (although it seemed like an amazing place to learn new things).

What have you gained from doing your PIPS?

I have become more confident with working with large number of samples and have also learnt how to prioritise the experiments and to perform them as efficiently as possible. In addition, I have learnt how to use AKTA protein purification system and performing CRISPR complex assembly and Cas protein activity assays (which are performed in RNAase-free box).

I also gained invaluable experience in working as part of a small biotech company and learnt first-hand the importance of collaboration and teamwork. To ensure that projects were finished according to the deadlines, experiments were efficiently redesigned and redistributed to several staff members. I learned that great communication and flexibility with your team members is extremely important, as these people also teach you new techniques and can also help you to troubleshoot.

How would you sum up your PIPS experience?

Of course, pandemic has made it a bit more difficult to travel, but I am glad I did my PIPS in CasZyme as it was great experience. I have learnt a lot of new techniques and developed friendly relationship with my colleagues. My primary laboratory manager planned the project in such a way that allowed me to learn as much as possible during my placement and made the internship comfortable during these difficult post-pandemic times. I really enjoyed being part of research team and part of CasZyme community.  Most of the staff were of my age, some also doing industrial PhDs. It was great to share the experience and support each other.

What advice would you give to other PGRs about PIPS?

Don’t be afraid to explore options outside your project (and comfort zone)! PIPS are aimed to help you learn new things, and add extra skills on your CV. I would personally recommend testing yourself in industry to see if you could work in a team with other people. The experience is also beneficial to see how industrial projects are managed and how things are generally organised (it might be quite different from your lab). I would recommend starting in a relatively small industrial company, as usually these biotechs are highly collaborative with academia and thus the working environment is slightly closer to the PhD in academia. Also, it means that you will be able to meet most of the staff during the placement and learn from them as much as possible.

Amy Stonadge (nee Brown) is a PhD student at the University of York who took part in a 3-month placement in the new COVID-19 LAMP Labs at the University of York. The LAMP Labs are a new collaboration between the University, Capita and the NHS and are working to establish a COVID-19 testing laboratory for frontline NHS staff. Read on to find out more about Amy’s PIPS experience!

Read moreAmy Stonadge (nee Brown) – LAMP Labs