Who it is for
This course is optional for White Rose BBSRC DTP students, including i-CASE. Maximum 15 places. Priority will be given to first years (and those on the waiting list from last year).
However, do not let this put you off applying – a waiting list will be held in case of last minute drop-outs.
If you have already informally expressed an interest in this course, please ensure you complete the formal booking form as soon as possible – at the bottom of this page.
When and where
This is a 4-day course from Tuesday 12th November to Friday 15th November 2019.
It will be held across all three universities within the White Rose BBSRC DTP, as follows:
DAY 1: Tuesday 12th November 2019
Start: 09:30 Finish 17:30-18:00 hours and then straight to pizza and drinks!
Venue: The University of York – Report to the Dept of Biology, Main Atrium
The evening social – including food – is for everyone!
Overnight accommodation will be provided in York for Leeds and Sheffield students.
DAY 2: Wednesday 13th November
Start: 09:15 Finish: 17:00 hours
Venue: The University of York – Report to the Dept of Biology, Main Atrium
DAY 3: Thursday 14th November 2019
Start: 09:30 Finish: 16:00 hours
Venue: The University of Leeds – Worsley Seminar Room (8.11G)
DAY 4: Friday 15th November 2019
Start: 09:30 Finish: 16:15 hours
Venue: The University of Sheffield – Meet in the Foyer of Firth Court
What you will do
You will gain knowledge and hands-on experience of modern confocal techniques.
Please ensure you arrive on time for the start times each day. The timings during each day may vary according to the needs of the group.
DAY 1 – York – From basic techniques through to advanced confocal microscopy
| 09:15 | Arrival and registration |
| 09:30 | Lecture – Introduction to fluorescence and confocal microscopy |
| 10:30 | Coffee break |
| 11:00 | Practical – Basics of confocal microscopy |
| 12:30 | Lunch |
| 13:15 | Practical – Multicolour imaging |
| 14:45 | Coffee break |
| 15:15 | Practical – z-stacks |
| 16:30 | Lecture – Beyond confocal |
| 17:30 | Social – Pizza and drinks |
DAY 2 – York – From basic techniques through to advanced confocal microscopy
| 09:00 | Arrival and registration – Note this is an earlier start time than Day 1 |
| 09:15 | Lecture – Fluorescent proteins, spectral unmixing and FRAP |
| 10:15 | Practical – Slow FRAP |
| 11:15 | Coffee break |
| 11:45 | Practical – Fast FRAP |
| 12:45 | Lunch |
| 13:30 | Practical – Spectral unmixing |
| 14:30 | Coffee break |
| 15:00 | Practical – Spectral unmixing, tiling and more |
| 17:00 | Finish |
DAY 3 – Leeds – Super-resolution
| 09:30 | Arrival and registration |
| 10:10 | Introduction |
| 10:20 | Resolution and Introduction to Super-Resolution |
| 10:40 | Airyscan |
| 11:00 | Coffee break |
| 11:10 | iSIM/SIM/STED |
| 11:50 | PALM/STORM |
| 12:20 | Lunch |
| 13:30 | Microscope Demonstrations (Bioimaging facility – Miall 5.08) |
| 15:00 | Coffee break |
| 15:30 | Discussion and closing remarks |
| 16:00 | Finish |
DAY 4 – Sheffield – Lightsheet and image analysis
| 09:30 | Arrival and registration |
| 09:45 | Coffee and Introduction (BMS Seminar Room 1) |
| 10:00 | Lightsheet Seminar (BMS Seminar Room 1) |
| 11:00 | Sample mounting demo/practical |
| 11:30 | Zeiss lightsheet demo session |
| 13:15 | Lunch (BMS Seminar Room 1) |
| 14:15 | Image analysis with Fiji training session |
| 16:15 | Finish |
Transport and accommodation
You will need to make your own transport arrangements for each location and claim expenses back through your own University – Don’t forget to keep receipts.
Sheffield and Leeds students should bear in mind that they will be staying in hotel accommodation overnight in York on Tuesday 12th November 2019 so no travel will be required that evening nor the following morning.
Refreshments
Lunch will be provided each day.
If you have any dietary requirements, please ensure that you detail these in the booking form.
Cost – Free
How to book
The deadline was originally Friday 25th October 2019 but the course was quickly oversubscribed – with a maximum of 15 places – so please note that
BOOKINGS ARE NOW CLOSED
Contact
Contact details for each University location will be sent out with joining instructions.
If you have any general queries, please contact:
Catherine Liddle
White Rose BBSRC Doctoral Training Partnership Co-ordinator
Student Education Service | Doctoral College
Faculty of Biological Sciences
7.82 Irene Manton Building | University of Leeds | Leeds LS2 9JT
Tel +44 (0)113 343 6463 | Ext 36463
E-Mail: c.m.liddle@leeds.ac.uk
DTP website: https://www.whiterose-mechanisticbiology-dtp.ac.uk/