Where did you go and what did you do?
I completed my internship at the Leeds FBS Mass Spectrometry Facility, where I was primarily involved in a range of projects related to proteomics and intact mass spectrometry. My main responsibilities included sample preparation for proteomics analysis and overseeing the running and analysis of intact mass spectrometry samples as part of the facility’s service.
A key aspect of my role was method optimisation—I worked on refining protocols for a new column in the intact mass spectrometer, ensuring improved performance and reliability.
I was also involved in method development for a liquid handling robot, which aimed to automate sample preparation of proteomics samples, thereby increasing efficiency and consistency in laboratory workflows.
Outside of the lab, I was responsible for driving the application for our LEAF Bronze Award, which recognises laboratories implementing sustainable practices, and we were awarded the LEAF Bronze award recently. I also created a poster showcasing the facility’s capabilities and potential applications, which was presented at the Astbury Open Day 2024—and it was awarded first prize!
What made you want to do that particular PIPS?
Mass spectrometry is a powerful analytical tool with a wide range of applications. As I previously had only limited experience with running mass spec samples as part of my own PhD project, I was keen to learn more this technique, from sample preparation to data analysis, and gain some more hands-on experience.
Beyond the technical aspects, I was also interested in understanding how a research facility operates. This internship provided valuable insight into the day-to-day management, service provision, and method development processes that support a wide range of scientific projects in a variety of disciplines.
What skills have you gained from your PIPS?
From a technical standpoint, I gained hands-on experience in intact and proteomics mass spectrometry, whilst also improving my proficiency with commonly used analysis software, writing SOPs for other users to assist in their data analysis.
Furthermore, I gained experience in science communication through the design and presentation of a facility poster showcasing our capabilities and potential applications. Leading the application for the LEAF Bronze award also strengthened my project management skills.
Developing a method for the liquid handling robot required a lot of problem solving, and for both this, and many other aspects of my PIPs, I needed to be able to understand and apply a wide variety of information. This allowed me to build upon the skills developed during my PhD, but challenged me to employ these skills to digest a much broader range of material, on topics I was unfamiliar with.
What has been the impact (or likely future impact) of your PIPS?
Starting out in the mass spec facility was more challenging than I thought it would be, as I was moving from a lab where I was an ‘expert’ in my own project, to an environment where I had little to no technical expertise. This meant a steep learning curve! However, by being involved in running the facility and developing my technical skills dramatically improved my confidence, as I realised there were many transferrable skills that I had gained from my PhD which I could utilise during my placement in the facility.
I hope that my contributions, such as method optimisation, SOP development, and automation improvements, have streamlined workflows and will benefit future users. Leading the LEAF Bronze Award application also promoted sustainable lab practices with a lasting impact.
Most importantly, I’ve built lasting friendships with the amazing mass spec team, making this an incredibly rewarding experience both professionally and personally.
How would you sum up your PIPS experience?
Overall, my internship provided valuable hands-on experience in mass spectrometry, method development, sustainability initiatives, and scientific communication, allowing me to develop both my technical expertise and transferable skills.
What advice would you give to other PGRs about PIPS?
I would encourage other PGRs to choose a PIPS placement that challenges them and helps them develop new skills. It’s a great opportunity to gain hands-on experience in an area you’re interested in but may not have had formal training in.
Don’t be afraid to reach out to places that aren’t actively recruiting— many opportunities aren’t advertised, but asking around or just having chats in the corridor can lead to unexpected opportunities!