Application of high-resolution phosphoproteomics to reveal novel metabolic control mechanisms in Campylobacter jejuni

Campylobacter jejuni is the most common cause of food-borne gastroenteritis in the western world. Human infections result from consumption of contaminated chicken and the incidence has increased in recent years, including the emergence of multi-drug resistant campylobacters. New interventions are needed to reduce the numbers of the bacteria in the food-chain. We know that may essential proteins in bacteria are controlled by covalent modifications to their structure. This project will seek to understand the role of protein phosphorylation in the regulation of metabolism of C. jejuni using cutting-edge proteomics analyses employing high-resolution mass spectrometry combined with biochemical analysis of protein function. Our approach may allow the identification of new targets for anti-microbial drugs or other interventions to reduce the burden of Campylobacteriosis.